From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

نویسندگان

  • Jin Zhang
  • Ivan Khvorostov
  • Michael Teitell
چکیده

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.

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عنوان ژورنال:

دوره 282  شماره 

صفحات  -

تاریخ انتشار 2008